Simultaneous Determination of Angiotensin I-Converting Enzyme Inhibitory Peptides in Tryptic Casein Hydrolysate by High-Performance Liquid Chromatography Combined with a Replicate Heart-Cut Column-Swi
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- 作者：Xiao-Lin Zhu ; Toshiro Matsui ; Keisuke Watanabe ; Koso Shiraishi ; Tatsuro Ueki ; Kiyoshi Matsumoto
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2007
- 出版时间：October 17, 2007
- 年：2007
- 卷：55
- 期：21
- 页码：8367 - 8371
- 全文大小：594K
- 年卷期：v.55,no.21(October 17, 2007)
- ISSN：1520-5118
文摘
A replicate heart-cut column-switching HPLC method combined with two switching valves was newly developed for the simultaneous determination of three antihypertensive peptides (Ala-Phe, Tyr-Pro, and Trp-Tyr) in tryptic casein hydrolysate in one run-in assay. After a first separation on an octadecyl silane (ODS) column, heart-cuts of each peptide were individually separated on a subsequent analytical ODS column: 26% acetonitrile for Ala-Phe and Tyr-Pro (32% for Trp-Tyr) in 0.1% trifluoroacetic acid containing 10 mM sodium 1-octanesulfonate at 0.8 mL/min. Ala-Phe, Tyr-Pro, and Trp-Tyr in casein hydrolysate were determined within 70 min to be 0.377 ± 0.037 mg/g, 2.50 ± 0.26 mg/g, and 0.096 ± 0.008 mg/g, respectively.