Is Lipid Peroxidation Induced by the Aqueous Suspension of Fullerene C60 Nanoparticles in the Brains of Cyprinus carpio?

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• 作者：Naohide Shinohara ; Takeru Matsumoto ; Masashi Gamo ; Arisa Miyauchi ; Shigehisa Endo ; Yoshitaka Yonezawa ; Junko Nakanishi
• 刊名：Environmental Science & Technology
• 出版年：2009
• 出版时间：February 1, 2009
• 年：2009
• 卷：43
• 期：3
• 页码：948-953
• 全文大小：270K
• 年卷期：v.43,no.3(February 1, 2009)
• ISSN：1520-5851

文摘

This study aimed to clarify whether fullerene C₆₀ nanoparticles induced lipid peroxidation in Cyprinus carpio brains. A stable well-characterized aqueous suspension of C₆₀ nanoparticle (diameter: 50th and 95th percentiles, 36 and 95 nm respectively) with 0.1% Tween80 solution was prepared by bead milling. Lipid hydroperoxides (LPO) were measured in vitro in homogenized fish brain tissues containing 33 μg/mg-protein dispersed C₆₀ nanoparticles under light and dark conditions to verify the lipid peroxidation ability of C₆₀ and the interference of light exposure by using a commercial assay kit. The LPO concentration significantly increased under the light condition but not under the dark condition. This suggests that C₆₀ has the lipid peroxidation ability under light condition, and the light exposure that occurs during the dissection and preparation of fish brain samples containing C₆₀ for the LPO assay interferes with the measurements of the in vivo LPO levels. Therefore, dissection and assay in the in vivo experiment were conducted under a yellow lamp or dark condition to avoid the interference of light. Moreover, the result of the in vitro test suggests that the LPO assay with irradiation might be a good method for detecting C₆₀ in brain tissues. In the in vivo experiment, C. carpio was exposed to 4.5 mg/L nano C₆₀ suspension for 48 h, following which the brain LPO concentration was measured. In the in vivo experiment, no fish died or exhibited abnormal symptoms during exposure. LPO assay of the C. carpio brain samples confirmed the absence of lipid peroxidation after exposure to 4.5 mg/L aqueous C₆₀ nanoparticle suspension for 48 h. Additional LPO assay under irradiation showed that C₆₀ did not reach the brain.