Microcavity Array System for Size-Based Enrichment of Circulating Tumor Cells from the Blood of Patients with Small-Cell Lung Cancer
详细信息 查看全文
- 作者：Masahito Hosokawa ; Takayuki Yoshikawa ; Ryo Negishi ; Tomoko Yoshino ; Yasuhiro Koh ; Hirotsugu Kenmotsu ; Tateaki Naito ; Toshiaki Takahashi ; Nobuyuki Yamamoto ; Yoshihito Kikuhara ; Hisashige Kanbara ; Tsuyoshi Tanaka ; Ken Yamaguchi ; Tadashi Matsunaga
- 刊名：Analytical Chemistry
- 出版年：2013
- 出版时间：June 18, 2013
- 年：2013
- 卷：85
- 期：12
- 页码：5692-5698
- 全文大小：279K
- 年卷期：v.85,no.12(June 18, 2013)
- ISSN：1520-6882
文摘
In this study, we present a method for efficient enrichment of small-sized circulating tumor cells (CTCs) such as those found in the blood of small-cell lung cancer (SCLC) patients using a microcavity array (MCA) system. To enrich CTCs from whole blood, a microfabricated nickel filter with a rectangular MCA (104 cavities/filter) was integrated with a miniaturized device, allowing for the isolation of tumor cells based on differences in size and deformability between tumor and blood cells. The shape and porosity of the MCA were optimized to efficiently capture small tumor cells on the microcavities under low flow resistance conditions, while allowing other blood cells to effectively pass through. Under optimized conditions, approximately 80% of SCLC (NCI-H69 and NCI-H82) cells spiked in 1 mL of whole blood were successfully recovered. In clinical samples, CTCs were detectable in 16 of 16 SCLC patients. In addition, the number of leukocytes captured on the rectangular MCA was significantly lower than that on the circular MCA (p < 0.001), suggesting that the use of the rectangular MCA diminishes a considerable number of carryover leukocytes. Therefore, our system has potential as a tool for the detection of CTCs in small cell-type tumors and detailed molecular analyses of CTCs.