Subpicogram Per Milliliter Detection of Interleukins Using Silicon Photonic Microring Resonators and an Enzymatic Signal Enhancement Strategy

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• 作者：Jared T. Kindt ; Matthew S. Luchansky ; Abraham J. Qavi ; So-Hyun Lee ; Ryan C. Bailey
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：November 19, 2013
• 年：2013
• 卷：85
• 期：22
• 页码：10653-10657
• 全文大小：323K
• 年卷期：v.85,no.22(November 19, 2013)
• ISSN：1520-6882

文摘

The detection of biomolecules at ultralow (low to subpicogram per milliliter) concentrations and within complex, clinically relevant matrices is a formidable challenge that is complicated by limitations imposed by the Langmuir binding isotherm and mass transport, for surface-based affinity biosensors. Here we report the integration of an enzymatic signal enhancement scheme onto a multiplexable silicon photonic microring resonator detection platform. To demonstrate the analytical value of this combination, we simultaneously quantitated levels of the interleukins IL-2, IL-6, and IL-8 in undiluted cerebrospinal fluid in an assay format that is multiplexable, relatively rapid (90 min), and features a 3 order of magnitude dynamic range and a limit of detection 鈮? pg/mL. The modular nature of this assay and technology should lend itself broadly amenable to different analyte classes, making it a versatile tool for biomarker analysis in clinically relevant settings.