Enhanced Schwann Cell Attachment and Alignment Using One-Pot 鈥淒ual Click鈥?GRGDS and YIGSR Derivatized Nanofibers

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• 作者：Jukuan Zheng ; Dimitria Kontoveros ; Fei Lin ; Geng Hua ; Darrell H. Reneker ; Matthew L. Becker ; Rebecca K. Willits
• 刊名：Biomacromolecules
• 出版年：2015
• 出版时间：January 12, 2015
• 年：2015
• 卷：16
• 期：1
• 页码：357-363
• 全文大小：456K
• ISSN：1526-4602

文摘

Using metal-free click chemistry and oxime condensation methodologies, GRGDS and YIGSR peptides were coupled to random and aligned degradable nanofiber networks postelectrospinning in a one-pot reaction. The bound peptides are bioactive, as demonstrated by Schwann cell attachment and proliferation, and the inclusion of YIGSR with GRGDS alters the expression of the receptor for YIGSR. Additionally, aligned nanofibers act as a potential guidance cue by increasing the aspect ratio and aligning the actin filaments, which suggest that peptide-functionalized scaffolds would be useful to direct SCs for peripheral nerve regeneration.