Rapid Isolation and Purification of 1-Cyano-2-Hydroxy-3-Butene (Crambene) from Crambe abyssinica Seed Meal Using Immiscible Solvent Extraction and High-Performance Liquid Chromatography

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• 作者：Thomas E. Niedoborski ; Barbara P. Klein ; and Matthew A. Wallig
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2001
• 出版时间：August 2001
• 年：2001
• 卷：49
• 期：8
• 页码：3594 - 3599
• 全文大小：64K
• 年卷期：v.49,no.8(August 2001)
• ISSN：1520-5118

文摘

1-Cyano-2-hydroxy-3-butene (crambene) is a nitrile found in cruciferous vegetables that causessignificant upregulation of quinone reductase and glutathione S-transferases in vivo and in vitro,making it a likely candidate as a cancer chemopreventive compound. To investigate further theputative anticarcinogenic mechanisms of crambene, a compound of the highest possible purity isvital. Therefore, a rapid and effective method of purification of crambene is necessary to continuestudies of its beneficial health effects. A rapid method to isolate and purify natural crambene fromeither Crambe abyssinica (crambe) seed or commercially processed crambe seed meal was developedusing immiscible solvent extraction followed by high-performance liquid chromatography. Use ofthis methodology eliminated the need for time-consuming and relatively inefficient columnchromatography, improved extraction efficiency, and resulted in higher purity than previously usedmethodologies. Elimination of trace amounts of fatty acid residues, unachievable with previousmethodologies, also was accomplished.Keywords: Crambene; 1-cyano-2-hydroxy-3-butene; immiscible solvent extraction; crambe; cruciferous vegetables; chemoprotection, anticarcinogenic; high-performance liquid chromatography