Preparative HPLC Method for the Purification of Sulforaphane and Sulforaphane Nitrile from Brassica oleracea
详细信息 查看全文
- 作者：Nathan V. Matusheski ; Matthew A. Wallig ; John A. Juvik ; Barbara P. Klein ; Mosbah M. Kushad ; and Elizabeth H. Jeffery
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2001
- 出版时间：April 2001
- 年：2001
- 卷：49
- 期：4
- 页码：1867 - 1872
- 全文大小：72K
- 年卷期：v.49,no.4(April 2001)
- ISSN：1520-5118
文摘
An extraction and preparative HPLC method has been devised to simultaneously purify sulforaphaneand sulforaphane nitrile from the seed of Brassica oleracea var. italica cv. Brigadier. The seed wasdefatted with hexane, dried, and hydrolyzed in deionized water (1:9) for 8 h. The hydrolyzed seedmeal was salted and extracted with methylene chloride. The dried residue was redissolved in a 5%acetonitrile solution and washed with excess hexane to remove nonpolar contaminants. The aqueousphase was filtered through a 0.22-
m cellulose filter and separated by HPLC using a Waters PrepNova-Pak HR C-18 reverse-phase column. Refractive index was used to detect sulforaphane nitrile,and absorbance at 254 nm was used to detect sulforaphane. Peak identification was confirmed usinggas chromatography and electron-impact mass spectrometry. Each kilogram of extracted seed yieldedapproximately 4.8 g of sulforaphane and 3.8 g of sulforaphane nitrile. Standard curves were developedusing the purified compounds to allow quantification of sulforaphane and sulforaphane nitrile inbroccoli tissue using a rapid GC method. The methodology was used to compare sulforaphane andsulforaphane nitrile content of autolyzed samples of several broccoli varieties.Keywords: Brassica oleracea; glucosinolates; broccoli; sulforaphane; sulforaphane nitrile; isothiocyanate; HPLC
m cellulose filter and separated by HPLC using a Waters PrepNova-Pak HR C-18 reverse-phase column. Refractive index was used to detect sulforaphane nitrile,and absorbance at 254 nm was used to detect sulforaphane. Peak identification was confirmed usinggas chromatography and electron-impact mass spectrometry. Each kilogram of extracted seed yieldedapproximately 4.8 g of sulforaphane and 3.8 g of sulforaphane nitrile. Standard curves were developedusing the purified compounds to allow quantification of sulforaphane and sulforaphane nitrile inbroccoli tissue using a rapid GC method. The methodology was used to compare sulforaphane andsulforaphane nitrile content of autolyzed samples of several broccoli varieties.Keywords: Brassica oleracea; glucosinolates; broccoli; sulforaphane; sulforaphane nitrile; isothiocyanate; HPLC