Development of an Immobilized Ketoreductase for Enzymatic (R)-1-(3,5-Bis(trifluoromethyl)phenyl)ethanol Production

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• 作者：Hongmei Li ; Johannah Moncecchi ; Matthew D. Truppo
• 刊名：Organic Process Research & Development
• 出版年：2015
• 出版时间：July 17, 2015
• 年：2015
• 卷：19
• 期：7
• 页码：695-700
• 全文大小：341K
• ISSN：1520-586X

文摘

The development of an immobilized ketoreductase via covalent binding on resin EC-HFA has demonstrated that it is highly active and stable in organic solvents and can be recycled and reused many times in both batch mode and flow reactor mode. (R)-1-(3,5-Bis(trifluoromethyl)phenyl)ethanol, a key chiral intermediate in the synthesis of EMEND, was synthesized in 98% yield with >99% ee using the immobilized ketoreductase in a 50% hexanes/40% IPA/10% water solvent system. The immobilized ketoreductase has also been applied to the synthesis of various chiral alcohols.