Molecular Analysis of Carbohydrate−Antibody Interactions: Case Study Using a Bacillus anthracis Tetrasaccharide
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- 作者：Matthias A. Oberli ; Marco Tamborrini ; Yu-Hsuan Tsai ; Daniel B. Werz ; Tim Horlacher ; Alexander Adibekian ; Dominik Gauss ; Heiko M. Mller ; Gerd Pluschke ; Peter H. Seeberger
- 刊名：Journal of the American Chemical Society
- 出版年：2010
- 出版时间：August 4, 2010
- 年：2010
- 卷：132
- 期：30
- 页码：10239-10241
- 全文大小：173K
- 年卷期：v.132,no.30(August 4, 2010)
- ISSN：1520-5126
文摘
The process for selecting potent and effective carbohydrate antigens is not well-established. A combination of synthetic glycan microarray screening, surface plasmon resonance analysis, and saturation transfer difference NMR spectroscopy was used to dissect the antibody-binding surface of a carbohydrate antigen, revealing crucial binding elements with atomic-level detail. This analysis takes the first step toward uncovering the rules for structure-based design of carbohydrate antigens.