Comparison of the Bioactivity of Two Glucoraphanin Hydrolysis Products Found in Broccoli, Sulforaphane and Sulforaphane Nitrile
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- 作者：Nathan V. Matusheski and Elizabeth H. Jeffery
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2001
- 出版时间：December 2001
- 年：2001
- 卷：49
- 期：12
- 页码：5743 - 5749
- 全文大小：86K
- 年卷期：v.49,no.12(December 2001)
- ISSN：1520-5118
文摘
Epidemiological and laboratory studies suggest that dietary broccoli may prevent or delay a varietyof cancers. Broccoli and other crucifers contain a relatively unique family of secondary metabolitescalled glucosinolates. Glucoraphanin, the major glucosinolate in broccoli, is hydrolyzed by anendogenous plant myrosinase to form either the potent anticarcinogen sulforaphane (SF) orsulforaphane nitrile (SF nitrile). The bioactivities of SF and SF nitrile were compared in rats andin mouse hepatoma cells. Male, 4-week-old, Fischer 344 rats were administered SF or SF nitrile(200, 500, or 1000 
mol/kg) by gavage daily for 5 days. Hepatic, colonic mucosal, and pancreaticquinone reductase and glutathione S-transferase activities were induced by high doses of SF, butnot by SF nitrile. When Hepa 1c1c7 cells were exposed to increasing levels of each compound for 24h, quinone reductase showed a 3-fold maximal induction over control at 2.5 M SF and a 3.5-foldmaximal induction over control at 2000 M SF nitrile, the highest concentration tested. These resultsdemonstrate that SF nitrile is substantially less potent than SF as an inducing agent of phase IIdetoxification enzymes. Therefore, glucoraphanin hydrolysis directed toward the production of SFrather than SF nitrile could increase the potential chemoprotective effects of broccoli.Keywords: Broccoli; glucosinolates; sulforaphane; sulforaphane nitrile; isothiocyanate; quinonereductase; glutathione S-transferase; Brassica oleracea
mol/kg) by gavage daily for 5 days. Hepatic, colonic mucosal, and pancreaticquinone reductase and glutathione S-transferase activities were induced by high doses of SF, butnot by SF nitrile. When Hepa 1c1c7 cells were exposed to increasing levels of each compound for 24h, quinone reductase showed a 3-fold maximal induction over control at 2.5 M SF and a 3.5-foldmaximal induction over control at 2000 M SF nitrile, the highest concentration tested. These resultsdemonstrate that SF nitrile is substantially less potent than SF as an inducing agent of phase IIdetoxification enzymes. Therefore, glucoraphanin hydrolysis directed toward the production of SFrather than SF nitrile could increase the potential chemoprotective effects of broccoli.Keywords: Broccoli; glucosinolates; sulforaphane; sulforaphane nitrile; isothiocyanate; quinonereductase; glutathione S-transferase; Brassica oleracea