Potential Application of a Glucose-Transport-Deficient Mutant of Schizosaccharomyces pombe for Removing Gluconic Acid from Grape Must

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• 作者：Rafael A. Peinado ; Juan J. Moreno ; Manuel Medina ; and Juan C. Mauricio
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2005
• 出版时间：February 23, 2005
• 年：2005
• 卷：53
• 期：4
• 页码：1017 - 1021
• 全文大小：51K
• 年卷期：v.53,no.4(February 23, 2005)
• ISSN：1520-5118

文摘

Musts from rotten grapes typically contain high levels of gluconic acid, which can raise severe problemsin winemaking processes. In this work, the ability of the glucose-transport-deficient mutant YGS-5 ofSchizosaccharomyces pombe to completely or partly remove gluconic acid from a synthetic glucose-containing medium and the potential use of this yeast strain for the same purpose in musts andwines were examined. Surprisingly, the S. pombe YGS-5 strain successfully removed 93% of theinitial gluconic acid (2.5 gL^-1) and 80% of the initial malic acid (1.0 gL^-1) within 30 h after inoculation.Also, the yeast strain produced no volatile compounds other than those obtained in fermentationsconducted with the wine yeast Saccharomyces cerevisiae. S. pombe YGS-5 could thus be used toremove gluconic acid present in musts from rotten grapes. On the basis of these results, variousways of using S. pombe YGS-5 to treat musts containing gluconic acid in order to solve the problemsdue to the high gluconic acid concentrations in botrytized grape must are proposed.Keywords: Gluconic acid; glucose-transport-deficient mutant; Schizosaccharomyces pombe; volatilecompounds