Nuclear Magnetic Resonance-Based Metabolic Comparative Analysis of Two Apple Varieties with Different Resistances to Apple Scab Attacks

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• 作者：Fabio Sciubba ; Maria Enrica Di Cocco ; Raffaella Gianferri ; Giorgio Capuani ; Flavio Roberto De Salvador ; Marco Fontanari ; Daniela Gorietti ; Maurizio Delfini
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：September 23, 2015
• 年：2015
• 卷：63
• 期：37
• 页码：8339-8347
• 全文大小：591K
• ISSN：1520-5118

文摘

Apple scab, caused by the fungus Venturia inaequalis, is the most serious disease of the apple worldwide. Two cultivars (Malus domestica), having different degrees of resistance against fungi attacks, were analyzed by ¹H and ¹³C nuclear magnetic resonance (NMR) spectroscopy. Aqueous and organic extracts of both apple flesh and skin were studied, and over 30 metabolites, classified as organic acids, amino acids, carbohydrates, phenolic compounds, lipids, sterols, and other metabolites, were quantified by means of one-dimensional (1D) and two-dimensional (2D) NMR experiments. The metabolic profiles of the two apple cultivars were compared, and the differences were correlated with the different degrees of resistance to apple scab by means of univariate analysis. Levels of metabolites with known antifungal activity were observed not only to be higher in the Almagold cultivar but also to show different correlation patterns in comparison to Golden Delicious, implying a difference in the metabolic network involved in their biosynthesis.