Measurement of Beryllium in Biological Samples by Accelerator Mass Spectrometry: Applications for Studying Chronic Beryllium Disease
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- 作者：Marina L. Chiarappa-Zucca ; Robert C. Finkel ; Roger E. Martinelli ; Jeffery E. McAninch ; David O. Nelson ; and Kenneth W. Turteltaub
- 刊名：Chemical Research in Toxicology
- 出版年：2004
- 出版时间：December 2004
- 年：2004
- 卷：17
- 期：12
- 页码：1614 - 1620
- 全文大小：130K
- 年卷期：v.17,no.12(December 2004)
- ISSN：1520-5010
文摘
A method using accelerator mass spectrometry (AMS) has been developed for quantifyingattomoles of beryllium (Be) in biological samples. This method provides the sensitivity to traceBe in biological samples at very low doses with the purpose of identifying the molecular targetsinvolved in chronic beryllium disease. Proof of the method was tested by administering 0.001,0.05, 0.5, and 5.0 
g of 9Be and 10Be by intraperitoneal injection to male mice and removingthe spleen, liver, femurs, blood, lungs, and kidneys after 24 h of exposure. These samples wereprepared for AMS analysis by tissue digestion in nitric acid, followed by further organicoxidation with hydrogen peroxide and ammonium persulfate and, last, precipitation of Be withammonium hydroxide and conversion to beryllium oxide at 800 
C. The 10Be/9Be ratio of theextracted beryllium oxide was measured by AMS, and Be in the original sample was calculated.Results indicate that Be levels were dose-dependent in all tissues and the highest levels weremeasured in the spleen and liver. The measured 10Be/9Be ratios spanned 4 orders of magnitude,from 10-10 to 10-14, with a detection limit of 3.0 × 10-14, which is equivalent to 0.8 amol of10Be. These results show that routine quantification of nanogram levels of Be in tissues ispossible and that AMS is a sensitive method that can be used in biological studies to understandthe molecular dosimetry of Be and mechanisms of toxicity.
g of 9Be and 10Be by intraperitoneal injection to male mice and removingthe spleen, liver, femurs, blood, lungs, and kidneys after 24 h of exposure. These samples wereprepared for AMS analysis by tissue digestion in nitric acid, followed by further organicoxidation with hydrogen peroxide and ammonium persulfate and, last, precipitation of Be withammonium hydroxide and conversion to beryllium oxide at 800 C. The 10Be/9Be ratio of theextracted beryllium oxide was measured by AMS, and Be in the original sample was calculated.Results indicate that Be levels were dose-dependent in all tissues and the highest levels weremeasured in the spleen and liver. The measured 10Be/9Be ratios spanned 4 orders of magnitude,from 10-10 to 10-14, with a detection limit of 3.0 × 10-14, which is equivalent to 0.8 amol of10Be. These results show that routine quantification of nanogram levels of Be in tissues ispossible and that AMS is a sensitive method that can be used in biological studies to understandthe molecular dosimetry of Be and mechanisms of toxicity.