One-Pot Synthesis, Purification, and Formulation of Bionanoparticle-CpG Oligodeoxynucleotide Hepatitis B Surface Antigen Conjugate Vaccine via Tangential Flow Filtration
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- 作者：Krishnaswami Raja ; Ross McDonald ; Stephen Tuck ; Roberto Rodriguez ; Bob Milley ; Paula Traquina
- 刊名：Bioconjugate Chemistry
- 出版年：2007
- 出版时间：March 2007
- 年：2007
- 卷：18
- 期：2
- 页码：285 - 288
- 全文大小：154K
- 年卷期：v.18,no.2(March 2007)
- ISSN：1520-4812
文摘
The synthesis and characterization of a Hepatitis B virus vaccine (HBsIC-ISS) candidate composed of HepatitisB surface antigen (HBsAg) bionanoparticles conjugated to multiple copies of immunostimulatory sequenceoligodeoxynucleotides is presented. An efficient tangential flow filtration (TFF) method has been developed topurify the conjugated bionanoparticles from the excess conjugation reagents. The TFF technique presented canserve as a rapid and convenient alternative to current methods like ultracentrifugation for the separation of excesssmall molecule/polymeric conjugation reagents from chemically modified viruses and other viruslike particles.