Multimodal Mass Spectrometry Imaging of N-Glycans and Proteins from the Same Tissue Section

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• 作者：Bram Heijs ; Stephanie Holst ; Inge H. Briaire-de Bruijn ; Gabi W. van Pelt ; Arnoud H. de Ru ; Peter A. van Veelen ; Richard R. Drake ; Anand S. Mehta ; Wilma E. Mesker ; Rob A. Tollenaar ; Judith V. M. G. Bovée ; Manfred Wuhrer ; Liam A. McDonnell
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：August 2, 2016
• 年：2016
• 卷：88
• 期：15
• 页码：7745-7753
• 全文大小：620K
• 年卷期：0
• ISSN：1520-6882

文摘

On-tissue digestion matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) can be used to record spatially correlated molecular information from formalin-fixed, paraffin-embedded (FFPE) tissue sections. In this work, we present the in situ multimodal analysis of N-linked glycans and proteins from the same FFPE tissue section. The robustness and applicability of the method are demonstrated for several tumors, including epithelial and mesenchymal tumor types. Major analytical aspects, such as lateral diffusion of the analyte molecules and differences in measurement sensitivity due to the additional sample preparation methods, have been investigated for both N-glycans and proteolytic peptides. By combining the MSI approach with extract analysis, we were also able to assess which mass spectral peaks generated by MALDI-MSI could be assigned to unique N-glycan and peptide identities.