Adsorption of Pathogenic Prion Protein to Quartz Sand
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- 作者：Xin Ma ; Craig H. Benson ; Debbie McKenzie ; Judd M. Aiken ; Joel A. Pedersen
- 刊名：Environmental Science & Technology
- 出版年：2007
- 出版时间：April 1, 2007
- 年：2007
- 卷：41
- 期：7
- 页码：2324 - 2330
- 全文大小：157K
- 年卷期：v.41,no.7(April 1, 2007)
- ISSN：1520-5851
文摘
Management responses to prion diseases of cattle, deer,and elk create a significant need for safe and effectivedisposal of infected carcasses and other materials.Furthermore, soil may contribute to the horizontal transmissionof sheep scrapie and cervid chronic wasting disease byserving as an environmental reservoir for the infectious agent.As an initial step toward understanding prion mobility inporous materials such as soil and landfilled waste, theinfluence of pH and ionic strength (I) on pathogenic prionprotein (PrPSc) properties (viz. aggregation state and
-potential) and adsorption to quartz sand was investigated.The apparent average isoelectric point of PrPSc aggregateswas 4.6. PrPSc aggregate size was largest between pH4 and 6, and increased with increasing I at pH 7. Adsorptionto quartz sand was maximal near the apparent isoelectricpoint of PrPSc aggregates and decreased as pH eitherdeclined or increased. PrPSc adsorption increasedas suspension I increased, and reached an apparentplateau at I ~ 0.1 M. While trends with pH and I in PrPScattachment to quartz surfaces were consistent withpredictions based on Born-DLVO theory, non-DLVO forcesappeared to contribute to adsorption at pH 7 and 9 (I =10 mM). Our findings suggest that disposal strategies thatelevate pH (e.g., burial in lime or fly ash), may increasePrPSc mobility. Similarly, PrPSc mobility may increase as alandfill ages, due to increases in pH and decreases in Iof the leachate.
-potential) and adsorption to quartz sand was investigated.The apparent average isoelectric point of PrPSc aggregateswas 4.6. PrPSc aggregate size was largest between pH4 and 6, and increased with increasing I at pH 7. Adsorptionto quartz sand was maximal near the apparent isoelectricpoint of PrPSc aggregates and decreased as pH eitherdeclined or increased. PrPSc adsorption increasedas suspension I increased, and reached an apparentplateau at I ~ 0.1 M. While trends with pH and I in PrPScattachment to quartz surfaces were consistent withpredictions based on Born-DLVO theory, non-DLVO forcesappeared to contribute to adsorption at pH 7 and 9 (I =10 mM). Our findings suggest that disposal strategies thatelevate pH (e.g., burial in lime or fly ash), may increasePrPSc mobility. Similarly, PrPSc mobility may increase as alandfill ages, due to increases in pH and decreases in Iof the leachate.