Identification of the Minimal Copper(II)-Binding α-Synuclein Sequence

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• 作者：Mark S. Jackson ; Jennifer C. Lee
• 刊名：Inorganic Chemistry
• 出版年：2009
• 出版时间：October 5, 2009
• 年：2009
• 卷：48
• 期：19
• 页码：9303-9307
• 全文大小：757K
• 年卷期：v.48,no.19(October 5, 2009)
• ISSN：1520-510X

文摘

Parkinson’s disease has been long linked to environmental factors, such as transition metals and recently to α-synuclein, a presynaptic protein. Using tryptophan-containing peptides, we identified the minimal Cu(II)-binding sequence to be within the first four residues, MDV(F/W), anchored by the α-amino terminus. In addition, mutant peptide 1−10 (Lys → Arg) verified that neither Lys6 nor Lys10 are necessary for Cu(II) binding. Interestingly, Trp4 excited-state decay kinetics measured for peptides and proteins reveal two quenching modes, possibly arising from two distinct Cu(II)−polypeptide structures.