Fast Stiffness Mapping of Cells Using High-Bandwidth Atomic Force Microscopy

详细信息    查看全文

• 作者：Andrew Wang ; Karthik Vijayraghavan ; Olav Solgaard ; Manish J. Butte
• 刊名：ACS Nano
• 出版年：2016
• 出版时间：January 26, 2016
• 年：2016
• 卷：10
• 期：1
• 页码：257-264
• 全文大小：687K
• ISSN：1936-086X

文摘

The cytoskeleton controls cellular morphology and mediates the mechanical interactions between a cell and its environment. Atomic force microscopy (AFM) has the unique capability to map cytoskeletal mechanics and structures with nanometer resolution. However, whole-cell cytomechanical imaging with conventional AFM techniques is limited by low imaging speed. Here, we present fast nanomechanical mapping of cells using high-bandwidth AFM (HB-AFM), where >10⁶ nanoindentation measurements were acquired in ～10 min—a task that would take weeks to finish using conventional AFM. High-bandwidth measurements enabled capture of the entire tip–sample interaction for each tap on cells, engendering a new measurement (“force phase”) that exceeds the contrast of conventional tapping mode and enabling spectral visualization of >10 harmonics. The abundance of measurements allowed discovery of subtle cytomechanical features, including the stiffness of fibers of the cellular spectrin network in situ. This approach bridges HB-AFM and high-harmonic imaging and opens future opportunities for measuring the dynamic mechanical properties of living cells.