Biotin鈭捨?Cyclodextrin: A New Host鈥揋uest System for the Immobilization of Biomolecules

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• 作者：Michael Holzinger ; Meenakshi Singh ; Serge Cosnier
• 刊名：Langmuir
• 出版年：2012
• 出版时间：August 28, 2012
• 年：2012
• 卷：28
• 期：34
• 页码：12569-12574
• 全文大小：360K
• 年卷期：v.28,no.34(August 28, 2012)
• ISSN：1520-5827

文摘

The formation of stable supramolecular interactions between biotin and 尾-cyclodextrin was studied. An association constant of 3 脳 10² M^鈥? could be determined by NMR measurements by mapping the high field shift differences of the 尾-cyclodextrin protons (H-3) at different biotin concentrations. With the aim to demonstrate a new alternative for the immobilization of bioreceptors, biotin and 尾-cyclodextrin tagged biomolecules were immobilized on transducer surfaces, which were functionalized with the correspondent host鈥揼uest partner. The reliability of this new affinity system was investigated using two enzymes (glucose oxidase and polyphenol oxidase) as biomolecule models. This supramolecular inclusion complex shows clear advantages to the classic biotin-(strept)avidin鈥揵iotin system due to a detrimental effect of the additional avidin layer reducing the transduction efficiency. A 7-fold increase in the maximum current density and an almost 20 times higher sensitivity were exhibited by the immobilized biological layer obtained using this new host鈥揼uest system.