Polyphenol Oxidase Containing Sidestreams as Emulsifiers of Rumen Bypass Linseed Oil Emulsions: Interfacial Characterization and Efficacy of Protection against in Vitro Ruminal Biohydrogenation

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• 作者：Frederik Gadeyne ; Nympha De Neve ; Bruno Vlaeminck ; Erik Claeys ; Paul Van der Meeren ; Veerle Fievez
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：May 18, 2016
• 年：2016
• 卷：64
• 期：19
• 页码：3749-3759
• 全文大小：479K
• 年卷期：0
• ISSN：1520-5118

文摘

The low transfer in ruminants of dietary polyunsaturated fatty acids to the milk or peripheral tissues is largely due to ruminal biohydrogenation. Lipids emulsified by a polyphenol oxidase (PPO) rich protein extract of red clover were shown before to be protected against this breakdown after cross-linking with 4-methylcatechol. Protein extracts of 13 other vegetal resources were tested. Surprisingly, the effectiveness to protect emulsified lipids against in vitro ruminal biohydrogenation largely depended on the origin of the extract and its protein concentration but was not related to PPO activity. Moreover, PPO isoforms in vegetal sources, effectively protecting emulsified lipids, were diverse and their presence at the emulsion interface did not seem essential. Potato tuber peels were identified as an interesting biological source of emulsifying proteins and PPO, particularly since protein extracts of industrial potato sidestreams proved to be suitable for the current application.