Convective Assembly of Bacteria for Surface-Enhanced Raman Scattering

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• 作者：Mehmet Kahraman ; M. Mü ; ge Yazici ; Fikrettin //pubs.acs.org/images/entities/Scedil.gif" border="0">ahin ; Mustafa &Ccedil ; ulha
• 刊名：Langmuir
• 出版年：2008
• 出版时间：February 5, 2008
• 年：2008
• 卷：24
• 期：3
• 页码：894 - 901
• 全文大小：1169K
• 年卷期：v.24,no.3(February 5, 2008)
• ISSN：1520-5827

文摘

A sample preparation method based on convective assembly for "whole-microorganism" identification using surface-enhanced Raman scattering (SERS) is developed. With this technique, a uniform sample can easily be prepared withsilver nanoparticles. During the deposition process, bacteria and nanoparticles are assembled to form a unique well-ordered structure with great reproducibility. The SERS spectra acquired from the samples prepared with this techniquehave better quality and improved reproducibility for SERS spectra obtained from the same sample and limited variationdue to the consistent sample preparation. E. coli, a Gram-negative bacilli, and Staphylococcus cohnii, a Gram-positivecoccus, are studied as model bacteria.