Replacement of Thymidine by a Modified Base in the Escherichia coli Genome

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• 作者：Angad P. Mehta ; Han Li ; Sean A. Reed ; Lubica Supekova ; Tsotne Javahishvili ; Peter G. Schultz
• 刊名：Journal of the American Chemical Society
• 出版年：2016
• 出版时间：June 15, 2016
• 年：2016
• 卷：138
• 期：23
• 页码：7272-7275
• 全文大小：280K
• 年卷期：0
• ISSN：1520-5126

文摘

Prokaryotic and eukaryotic genomic DNA is comprised of the four building blocks A, G, C, and T. We have begun to explore the consequences of replacing a large fraction or all of a nucleoside in genomic DNA with a modified nucleoside. As a first step we have investigated the possibility of replacement of T by 2′-deoxy-5-(hydroxymethyl)uridine (5hmU) in the genomic DNA of Escherichia coli. Metabolic engineering with phage genes followed by random mutagenesis enabled us to achieve approximately 75% replacement of T by 5hmU in the E. coli genome and in plasmids.