Biofunctionalization of a 鈥淐lickable鈥?Organic Layer Photochemically Grafted on Titanium Substrates
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- 作者：Yan Li ; Meirong Zhao ; Jun Wang ; Kai Liu ; Chengzhi Cai
- 刊名：Langmuir
- 出版年：2011
- 出版时间：April 19, 2011
- 年：2011
- 卷：27
- 期：8
- 页码：4848-4856
- 全文大小：994K
- 年卷期：v.27,no.8(April 19, 2011)
- ISSN：1520-5827
文摘
We have developed a general method combining photochemical grafting and copper-catalyzed click chemistry for biofunctionalization of titanium substrates. The UV-activated grafting of an 伪,蠅-alkenyne onto TiO2/Ti substrates provided a 鈥渃lickable鈥?thin film platform. The selective attachment of the vinyl end of the molecule to the surface was achieved by masking the alkynyl end with a trimethylgermanyl (TMG) protecting group. Subsequently, various oligo(ethylene glycol) (OEG) derivatives terminated with an azido group were attached to the TMG-alkynyl modified titanium surface via a one-pot deprotection/click reaction. The films were characterized by X-ray photoelectron spectroscopy (XPS), contact angle goniometry, ellipsometry, and atomic force microscopy (AFM). We showed that the titanium surface presenting click-immobilized OEG substantially suppressed the nonspecific attachment of protein and cells as compared to the unmodified titanium substrate. Furthermore, glycine-arginine-glycine-aspartate (GRGD), a cell adhesion peptide, was coimmobilized with OEG on the platform. We demonstrated that the resultant GRGD-presenting thin film on Ti substrates can promote the specific adhesion and spreading of AsPC-1 cells.