Inhibition of Aflatoxin Biosynthesis in Aspergillus flavus by Diferuloylputrescine and p-Coumaroylferuloylputrescine
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- 作者：Jay E. Mellon and Robert A. Moreau
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2004
- 出版时间：October 20, 2004
- 年：2004
- 卷：52
- 期：21
- 页码：6660 - 6663
- 全文大小：51K
- 年卷期：v.52,no.21(October 20, 2004)
- ISSN：1520-5118
文摘
A mixture of diferuloylputrescine/p-coumaroylferuloylputrescine (85:15, w/w) demonstrated inhibitoryactivity against aflatoxin B1 biosynthesis in Aspergillus flavus isolate AF13. Inhibition was concentrationdependent, with a 50% effective dose of 30 
g of diferuloylputrescine/p-coumaroylferuloylputrescineper milliliter of medium. Aflatoxin inhibition levels of up to 93% were achieved using this conjugatedpolyamine material. This diconjugated polyamine mixture did not display inhibitory effects on A. flavusgrowth (mycelial weight) at any of the concentrations tested. A survey of hand-dissected corn (Zeamays) kernel tissues, including endosperm, germ, pericarp, and wax, revealed that the highestconcentrations of these conjugated polyamines were localized in the pericarp of the seed. Analysisof a number of corn accessions did not reveal a correlation between diferuloylputrescine/p-coumaroylferuloylputrescine concentration and resistance/susceptibility to A. flavus infection. Thelocalization of these diconjugated polyamine components in the pericarp, which functions as a physicalbarrier and surrounds the internal food storage reserves, suggests a defensive role for these materials.Keywords: Aflatoxin; Aspergillus flavus; corn; diferuloylputrescine; p-coumaroylferuloylputrescine;pericarp; Zea mays
g of diferuloylputrescine/p-coumaroylferuloylputrescineper milliliter of medium. Aflatoxin inhibition levels of up to 93% were achieved using this conjugatedpolyamine material. This diconjugated polyamine mixture did not display inhibitory effects on A. flavusgrowth (mycelial weight) at any of the concentrations tested. A survey of hand-dissected corn (Zeamays) kernel tissues, including endosperm, germ, pericarp, and wax, revealed that the highestconcentrations of these conjugated polyamines were localized in the pericarp of the seed. Analysisof a number of corn accessions did not reveal a correlation between diferuloylputrescine/p-coumaroylferuloylputrescine concentration and resistance/susceptibility to A. flavus infection. Thelocalization of these diconjugated polyamine components in the pericarp, which functions as a physicalbarrier and surrounds the internal food storage reserves, suggests a defensive role for these materials.Keywords: Aflatoxin; Aspergillus flavus; corn; diferuloylputrescine; p-coumaroylferuloylputrescine;pericarp; Zea mays