New Generation of Ensemble-Decision Aliquot Ranking Based on Simplified Microfluidic Components for Large-Capacity Trapping of Circulating Tumor Cells
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- 作者：Mengxia Zhao ; Wyatt C. Nelson ; Bingchuan Wei ; Perry G. Schiro ; Bejan M. Hakimi ; Eleanor S. Johnson ; Robbyn K. Anand ; Grace S. Gyurkey ; Lisa M. White ; Samuel H. Whiting ; Andrew L. Coveler ; Daniel T. Chiu
- 刊名：Analytical Chemistry
- 出版年：2013
- 出版时间：October 15, 2013
- 年：2013
- 卷：85
- 期：20
- 页码：9671-9677
- 全文大小：318K
- 年卷期：v.85,no.20(October 15, 2013)
- ISSN：1520-6882
文摘
Ensemble-decision aliquot ranking (eDAR) is a sensitive and high-throughput method to analyze circulating tumor cells (CTCs) from peripheral blood. Here, we report the next generation of eDAR, where we designed and optimized a new hydrodynamic switching scheme for the active sorting step in eDAR, which provided fast cell sorting with an improved reproducibility and stability. The microfluidic chip was also simplified by incorporating a functional area for subsequent purification using microslits fabricated by standard lithography method. Using the reported second generation of eDAR, we were able to analyze 1 mL of whole-blood samples in 12.5 min, with a 95% recovery and a zero false positive rate (n = 15).