High Viability of Cells Encapsulated in Degradable Poly(carboxybetaine) Hydrogels

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• 作者：Hsiu-Wen Chien ; Xuewei Xu ; Jean-Rene Ella-Menye ; Wei-Bor Tsai ; Shaoyi Jiang
• 刊名：Langmuir
• 出版年：2012
• 出版时间：December 21, 2012
• 年：2012
• 卷：28
• 期：51
• 页码：17778-17784
• 全文大小：377K
• 年卷期：v.28,no.51(December 21, 2012)
• ISSN：1520-5827

文摘

In this study, we report a degradable poly(carboxybetaine) (pCB) hydrogel, produced via a thiol鈥揹isulfide exchange reaction for cell encapsulation. A pCB dithiol was synthesized as a cross-linker and reacted with a pyridyl dithiol-containing CB copolymer to form a hydrogel. We evaluated the biocompatibility of the pCB-based hydrogel via encapsulation of three cell types, including NIH3T3 fibroblasts, MG63 osteoblast-like cells, and HepG2 hepatocarcinoma cells. Up to 90% of cells retained their viability in the pCB hydrogel even at low cell-seeding densities under serum-free conditions after a 9-day culture. Results are compared with a degradable poly(ethylene glycol) methacrylate (PEGMA) hydrogel, which showed very low cell viability under serum-free condition after a 3-day culture. We incorporated an RGD peptide into the CB hydrogel using a cysteine-terminated cross-linker, which was shown to promote cell proliferation.