High-Sensitivity Amperometric Biosensors Based on Ferrocene-Modified Linear Poly(ethylenimine)

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• 作者：Stephen A. Merchant ; Tu O. Tran ; Matthew T. Meredith ; Tracy C. Cline ; Daniel T. Glatzhofer ; David W. Schmidtke
• 刊名：Langmuir
• 出版年：2009
• 出版时间：July 7, 2009
• 年：2009
• 卷：25
• 期：13
• 页码：7736-7742
• 全文大小：821K
• 年卷期：v.25,no.13(July 7, 2009)
• ISSN：1520-5827

文摘

Amperometric biosensors for glucose and hydrogen peroxide have been built by immobilizing glucose oxidase (GOX) and horseradish peroxidase (HRP) in cross-linked films of ferrocene-modified linear poly(ethylenimine). At pH 7, the glucose sensors generated limiting catalytic current densities of 1.2 mA/cm². These current densities are approximately 4 times higher than those with other ferrocene-based redox polymers and are comparable to the highest reported values for osmium-based redox polymers with GOX. Because of the high sensitivity of these films (73 nA/cm²·μM), glucose concentrations in the micromolar range could be detected. Similarly, sensors were constructed with HRP-generated current densities of 0.9 mA/cm² under saturation conditions and sensitivities of 500 nA/cm²·μM. The results show that the ability of Fc-LPEI to effectively communicate with a variety of enzymes has potential applications in measuring low substrate concentrations in implantable biosensors and producing high current outputs in enzymatic biofuel cells.