High Throughput Analysis of Red Wine and Grape Phenolics-Adaptation and Validation of Methyl Cellulose Precipitable Tannin Assay and Modified Somers Color Assay to a Rapid 96 Well Plate Format
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- 作者：Meagan D. Mercurio ; Robert G. Dambergs ; Markus J. Herderich ; Paul A. Smith
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2007
- 出版时间：June 13, 2007
- 年：2007
- 卷：55
- 期：12
- 页码：4651 - 4657
- 全文大小：64K
- 年卷期：v.55,no.12(June 13, 2007)
- ISSN：1520-5118
文摘
The methyl cellulose precipitable (MCP) tannin assay and a modified version of the Somers andEvans color assay were adapted to high-throughput (HTP) analysis. To improve efficiency of theMCP tannin assay, a miniaturized 1 mL format and a HTP format using 96 well plates were developed.The Somers color assay was modified to allow the standardization of pH and ethanol concentrationsof wine samples in a simple one-step dilution with a buffer solution, thus removing inconsistenciesbetween wine matrices prior to analysis and allowing for its adaptation to a HTP format. Validationstudies showed that all new formats were efficient, and results were reproducible and analogous tothe original formats.