Salt-Dependent Conditional Protein Splicing of an Intein from Halobacterium salinarum

详细信息    查看全文

• 作者：Julie N. Reitter ; Christopher E. Cousin ; Michael C. Nicastri ; Mario V. Jaramillo ; Kenneth V. Mills
• 刊名：Biochemistry
• 出版年：2016
• 出版时间：March 8, 2016
• 年：2016
• 卷：55
• 期：9
• 页码：1279-1282
• 全文大小：283K
• ISSN：1520-4995

文摘

An intein from Halobacterium salinarum can be isolated as an unspliced precursor protein with exogenous exteins after Escherichia coli overexpression. The intein promotes protein splicing and uncoupled N-terminal cleavage in vitro, conditional on incubation with NaCl or KCl at concentrations of >1.5 M. The protein splicing reaction also is conditional on reduction of a disulfide bond between two active site cysteines. Conditional protein splicing under these relatively mild conditions may lead to advances in intein-based biotechnology applications and hints at the possibility that this H. salinarum intein could serve as a switch to control extein activity under physiologically relevant conditions.