Axial Nanometer Distances Measured by Fluorescence Lifetime Imaging Microscopy

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• 作者：Michael Berndt ; Mike Lorenz ; Jrg Enderlein ; Stefan Diez
• 刊名：Nano Letters
• 出版年：2010
• 出版时间：April 14, 2010
• 年：2010
• 卷：10
• 期：4
• 页码：1497-1500
• 全文大小：293K
• 年卷期：v.10,no.4(April 14, 2010)
• ISSN：1530-6992

文摘

We present a novel fluorescence lifetime imaging microscopy technique to measure absolute positions of fluorescent molecules within 100 nm above a metalized surface based on distance-dependent fluorescence lifetime modulations. We apply this technique to fluorescently labeled microtubules as optical probes with various unlabeled proteins attached. By measuring the fluorescence lifetimes, we obtain the position of the microtubules and therefore determine the geometrical size of the attached proteins with nanometer precision.