Three Minutes-Long Electrophoretically Assisted Zeptomolar Microfluidic Immunoassay with Magnetic-Beads Detection

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• 作者：Victor N. Morozov ; Stephanie Groves ; Michael J. Turell ; Charles Bailey
• 刊名：Journal of the American Chemical Society
• 出版年：2007
• 出版时间：October 24, 2007
• 年：2007
• 卷：129
• 期：42
• 页码：12628 - 12629
• 全文大小：134K
• 年卷期：v.129,no.42(October 24, 2007)
• ISSN：1520-5126

文摘

An ultrasensitive, two-step immunoassay method was developed for rapid assay of proteins and viruses. In the first step, electrophoresis was used to quickly bring soluble analytes from a flowing solution to a microarray of probe molecules immobilized on a semipermeable membrane. In the second step, the captured analyte was detected by scanning the microarray with functionalized magnetic beads passed over the array surface by shear flow and pressed to the array surface by a magnetic field. We demonstrated that this method routinely detected analytes in concentrations as low as 20 aM (500-1000 molecules, or 1-2 zeptamoles) within 2-3 min in the presence of a ~10¹¹-fold excess of other protein molecules in the sample.