Rapid Isolation and Identification of Bacteriophage T4-Encoded Modifications of Escherichia coli RNA Polymerase: A Generic Method to Study Bacteriophage/Host Interactions

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• 作者：Lars F. Westblade ; Leonid Minakhin ; Konstantin Kuznedelov ; Alan J. Tackett ; Emmanuel J. Chang ; Rachel A. Mooney ; Irina Vvedenskaya ; Qing Jun Wang ; David Fenyö ; Michael P. Rout ; Robert Landick ; Br
• 刊名：Journal of Proteome Research
• 出版年：2008
• 出版时间：March 2008
• 年：2008
• 卷：7
• 期：3
• 页码：1244 - 1250
• 全文大小：620K
• 年卷期：v.7,no.3(March 2008)
• ISSN：1535-3907

文摘

Bacteriophages are bacterial viruses that infect bacterial cells, and they have developed ingenious mechanisms to modify the bacterial RNA polymerase. Using a rapid, specific, single-step affinity isolation procedure to purify Escherichia coli RNA polymerase from bacteriophage T4-infected cells, we have identified bacteriophage T4-dependent modifications of the host RNA polymerase. We suggest that this methodology is broadly applicable for the identification of bacteriophage-dependent alterations of the host synthesis machinery.