Two-Prong Inhibitors for Human Carbonic Anhydrase II

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• 作者：Bidhan C. Roy ; Abir L. Banerjee ; Michael Swanson ; Xiao G. Jia ; Manas K. Haldar ; Sanku Mallik ; and D. K. Srivastava
• 刊名：Journal of the American Chemical Society
• 出版年：2004
• 出版时间：October 20, 2004
• 年：2004
• 卷：126
• 期：41
• 页码：13206 - 13207
• 全文大小：48K
• 年卷期：v.126,no.41(October 20, 2004)
• ISSN：1520-5126

文摘

The enzyme inhibitors are usually designed by taking into consideration the overall dimensions of the enzyme's active site pockets. This conventional approach often fails to produce desirable affinities of inhibitors for their cognate enzymes. To circumvent such constraints, we contemplated enhancing the binding affinities of inhibitors by attaching tether groups, which would interact with the surface exposed amino acid residues. This strategy has been tested for the inhibition of human carbonic anhydrase II. Benzenesulfonamide serves as a weak inhibitor for the enzyme, but when it is conjugated to iminodiacetate-Cu²⁺ (which interacts with the surface-exposed His residues) via a spacer group, its binding affinity is enhanced by about 2 orders of magnitude. This "two-prong" approach is expected to serve as a general strategy for converting weak inhibitors of enzymes into tight-binding inhibitors.