Direct Synthesis of Aqueous Quantum Dots through 4,4鈥?Bipyridine-Based Twin Ligand Strategy

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• 作者：Mausam Kalita ; Sreeram Cingarapu ; Santanu Roy ; Seok Chan Park ; Daniel Higgins ; Ryszard Jankowiak ; Viktor Chikan ; Kenneth J. Klabunde ; Stefan H. Bossmann
• 刊名：Inorganic Chemistry
• 出版年：2012
• 出版时间：April 16, 2012
• 年：2012
• 卷：51
• 期：8
• 页码：4521-4526
• 全文大小：365K
• 年卷期：v.51,no.8(April 16, 2012)
• ISSN：1520-510X

文摘

We report a new class of derivatized 4,4鈥?bipyridinium ligands for use in synthesizing highly fluorescent, extremely stable, water-soluble CdSe and CdTe quantum dots (QDs) for bioconjugation. We employed an evaporation鈥揷ondensation technique, also known as solvated metal atom dispersion (SMAD), followed by a digestive ripening procedure. This method has been used to synthesize both metal nanoparticles and semiconductors in the gram scale with several stabilizing ligands in various solvents. The SMAD technique comprised evaporation condensation and stabilization of CdSe or CdTe in tetrahydrofuran. The as-prepared product was then digestively ripened in both water and dimethyl formamide, leading to narrowing of the particle size distributions. The ligands were synthesized by nucleophilic substitution (S_N2) reactions using 4,4鈥?bipyridine as a nucleophile. Confocal microscopy images revealed the orange color of the nanocrystalline QDs with diameters of 5 nm. The size has been confirmed by using transmission electron microscopy. As a part of our strategy, 85% of the 4,4鈥?bipyridinium salt was synthesized as propionic acid derivative and used to both stabilize the QDs in water and label basic amino acids and different biomarkers utilizing the carboxylic acid functional group. Fifteen percent of the 4,4鈥?bipyridinium salt was synthesized as N-propyl maleimide and used as a second ligand to label any protein containing the amino acid cysteine by means of a 1,4-Michael addition.