A Reproducible Surface-Enhanced Raman Spectroscopy Approach. Online SERS Measurements in a Segmented Microfluidic System
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- 作者：Katrin R. Strehle ; Dana Cialla ; Petra Rö ; sch ; Thomas Henkel ; Michael Kö ; hler ; Jü ; rgen Popp
- 刊名：Analytical Chemistry
- 出版年：2007
- 出版时间：February 15, 2007
- 年：2007
- 卷：79
- 期：4
- 页码：1542 - 1547
- 全文大小：229K
- 年卷期：v.79,no.4(February 15, 2007)
- ISSN：1520-6882
文摘
The application of a liquid/liquid microsegmented flowfor serial high-throughput microanalytical systems showspromising prospects for applications in clinical chemistry,pharmaceutical research, process diagnostics, and analytical chemistry. Microscopy and microspectral analyticsoffer powerful approaches for the analytical readout ofdroplet based assays. Within the generated segments,individuality and integrity are retained during the complete diagnostic process making the approach favored foranalysis of individual microscaled objects like cells andmicroorganisms embedded in droplets. Here we reporton the online application of surface-enhanced micro-Raman spectroscopy for the detection and quantizationof analytes in a liquid/liquid segmented microfluidicsystem. Data acquisition was performed in microsegmentsdown to a volume of 180 nl. With this approach, weovercome the well-known problem of adhesion of colloid/analyte conjugates to the optical windows of detectioncuvettes, which causes the so-called "memory effect". Thecombination of the segmented microfluidic system withthe highly sensitive SERS technique reaches in a reproducible quantification of analytes with the SERS technique.