Spatial and Temporal Control of Microwave Triggered Chemiluminescence: A Protein Detection Platform

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• 作者：Michael J. R. Previte ; Kadir Aslan ; Chris D. Geddes
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：September 15, 2007
• 年：2007
• 卷：79
• 期：18
• 页码：7042 - 7052
• 全文大小：358K
• 年卷期：v.79,no.18(September 15, 2007)
• ISSN：1520-6882

文摘

We have combined the principles of microwave circuitryand antenna design and our recent work in microwave-triggered metal-enhanced chemiluminescence to now"trigger" chemically and enzyme-catalyzed chemiluminescent reactions with spatial and temporal control. Withthis technology platform, we achieve spatial and temporalcontrol of enzyme and chemically catalyzed chemiluminescence reactions to achieve more than 500-fold increases in "on-demand" photon flux from chemicallycatalyzed chemiluminescent reactions. We also report a6-fold increase in photon flux from HRP-catalyzed assayson disposable coverslips functionalized with HRP andplaced proximal to the substrates modified with thin-filmaluminum triangle disjointed "bow-tie" structures. Inaddition, we demonstrate the applicability of this technology to develop multiplexed or high-throughput chemiluminescent assays. We also demonstrate the clinical andbiological relevance of this technology platform by affixingaluminum structures in proximity to HRP protein immobilized on nitrocellulose to improve the sensitivity forthis model Western blot scheme by 50-fold. We believeanalytical applications that rely on enzyme-catalyzed chemiluminescence, such as immunoassays, may greatly benefitfrom this new platform technology.