Live Cell Interferometry Reveals Cellular Dynamism During Force Propagation

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• 作者：Jason Reed ; Joshua J. Troke ; Joanna Schmit ; Sen Han ; Michael A. Teitell ; James K. Gimzewski
• 刊名：ACS Nano
• 出版年：2008
• 出版时间：May 2008
• 年：2008
• 卷：2
• 期：5
• 页码：841-846
• 全文大小：352K
• 年卷期：0
• ISSN：1936-086X

文摘

Cancer and many other diseases are characterized by changes in cell morphology, motion, and mechanical rigidity. However, in live cell cytology, stimulus-induced morphologic changes typically take 10−30 min to detect. Here, we employ live-cell interferometry (LCI) to visualize the rapid response of a whole cell to mechanical stimulation, on a time scale of seconds, and we detect cytoskeletal remodeling behavior within 200 s. This behavior involved small, rapid changes in cell content and miniscule changes in shape; it would be difficult to detect with conventional or phase contrast microscopy alone and is beyond the dynamic capability of AFM. We demonstrate that LCI provides a rapid, quantitative reconstruction of the cell body with no labeling. This is an advantage over traditional microscopy and flow cytometry, which require cell surface tagging and/or destructive cell fixation for labeling