Comprehensive Proteomic Analysis of Human Cervical-Vaginal Fluid
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- 作者：Surendra Dasari ; Leonardo Pereira ; Ashok P. Reddy ; John-Edward A. Michaels ; Xinfang Lu ; Thomas Jacob ; Archana Thomas ; Matthew Rodland ; Charles T. Roberts ; Jr. ; Michael G. Gravett ; Srinivasa R. Nagalla
- 刊名：Journal of Proteome Research
- 出版年：2007
- 出版时间：April 2007
- 年：2007
- 卷：6
- 期：4
- 页码：1258 - 1268
- 全文大小：186K
- 年卷期：v.6,no.4(April 2007)
- ISSN：1535-3907
文摘
Cervical-vaginal fluid (CVF) is a potential rich source of biomarkers for enhancing our understandingof human parturition and pathologic conditions affecting pregnancy. In this study, we performed acomprehensive survey of the CVF proteome in pregnancy utilizing multidimensional liquid chromatography (2D-LC) coupled with mass spectrometry and gel-electrophoresis-based protein separationand identification. In total, 150 unique proteins were identified using multiple protein identificationalgorithms. Metabolism (32%) and immune response-related (22%) proteins are the major functionalcategories represented in the CVF proteome. A comparison of the CVF, serum, and amniotic fluidproteomes showed that 77 proteins are unique to CVF, while 56 and 17 CVF proteins also occur inserum and amniotic fluid, respectively. This data set provides a foundation for evaluation of theseproteins as potential CVF biomarkers for noninvasive diagnosis of pregnancy-related disorders.