Separation of Methicillin-Resistant from Methicillin-Susceptible Staphylococcus aureus by Electrophoretic Methods in Fused Silica Capillaries Etched with Supercritical Water
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- 作者：Marie Hork谩 ; Pavel Kar谩sek ; Filip R暖啪i膷ka ; Milada Dvo艡谩膷kov谩 ; Martina Sittov谩 ; Michal Roth
- 刊名：Analytical Chemistry
- 出版年：2014
- 出版时间：October 7, 2014
- 年：2014
- 卷：86
- 期：19
- 页码：9701-9708
- 全文大小：378K
- ISSN：1520-6882
文摘
Identification and prevention of Staphylococcus aureus-caused infections may benefit from a fast and dependable method to distinguish between the methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) S. aureus strains. The current methods involving polymerase chain reaction and/or other molecular tests are usually laborious and time-consuming. We describe here a fast and low-cost method employing capillary zone electrophoresis (CZE) to distinguish between MRSA and MSSA. The method makes use of a supercritical water-treated fused silica capillary, the inner surface of which has subsequently been modified with (3-glycidyloxypropyl)trimethoxysilane. With optimized proportions of suitable additives to the background electrolyte, a CZE separation of MRSA from MSSA may be completed within 12 min. The cells were baseline-resolved, and resolution was determined to be 3.61. The isoelectric points of MSSA and MRSA were found to be the same for both groups of these strains, pI = 3.4.