Sensing Proteins through Nanopores: Fundamental to Applications

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• 作者：Abdelghani Oukhaled ; Laurent Bacri ; Manuela Pastoriza-Gallego ; Jean-Michel Betton ; Juan Pelta
• 刊名：ACS Chemical Biology
• 出版年：2012
• 出版时间：December 21, 2012
• 年：2012
• 卷：7
• 期：12
• 页码：1935-1949
• 全文大小：689K
• 年卷期：v.7,no.12(December 21, 2012)
• ISSN：1554-8937

文摘

Proteins subjected to an electric field and forced to pass through a nanopore induce blockades of ionic current that depend on the protein and nanopore characteristics and interactions between them. Recent advances in the analysis of these blockades have highlighted a variety of phenomena that can be used to study protein translocation and protein folding, to probe single-molecule catalytic reactions in order to obtain kinetic and thermodynamic information, and to detect protein鈥揳ntibody complexes, proteins with DNA and RNA aptamers, and protein鈥損ore interactions. Nanopore design is now well controlled, allowing the development of future biotechnologies and medicine applications.