Exploring the Plant–Microbe Interface by Profiling the Surface-Associated Proteins of Barley Grains

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• 作者：Abida Sultan ; Birgit Andersen ; Birte Svensson ; Christine Finnie
• 刊名：Journal of Proteome Research
• 出版年：2016
• 出版时间：April 1, 2016
• 年：2016
• 卷：15
• 期：4
• 页码：1151-1167
• 全文大小：495K
• ISSN：1535-3907

文摘

Cereal grains are colonized by a microbial community that actively interacts with the plant via secretion of various enzymes, hormones, and metabolites. Microorganisms decompose plant tissues by a collection of depolymerizing enzymes, including β-1,4-xylanases, that are in turn inhibited by plant xylanase inhibitors. To gain insight into the importance of the microbial consortia and their interaction with barley grains, we used a combined gel-based (2-DE coupled to MALDI-TOF-TOF MS) and gel-free (LC–MS/MS) proteomics approach complemented with enzyme activity assays to profile the surface-associated proteins and xylanolytic activities of two barley cultivars. The surface-associated proteome was dominated by plant proteins with roles in defense and stress-responses, while the relatively less abundant microbial (bacterial and fungal) proteins were involved in cell-wall and polysaccharide degradation and included xylanases. The surface-associated proteomes showed elevated xylanolytic activity and contained several xylanases. Integration of proteomics with enzyme assays is a powerful tool for analysis and characterization of the interaction between microbial consortia and plants in their natural environment.