Traceability of Italian Protected Designation of Origin (PDO) Table Olives by Means of Microsatellite Molecular Markers
详细信息 查看全文
- 作者：Antonella Pasqualone ; Valentina Di Rienzo ; Raffaella Nasti ; Antonio Blanco ; Tommaso Gomes ; Cinzia Montemurro
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2013
- 出版时间：March 27, 2013
- 年：2013
- 卷：61
- 期：12
- 页码：3068-3073
- 全文大小：226K
- 年卷期：v.61,no.12(March 27, 2013)
- ISSN：1520-5118
文摘
The aim of this work was to develop a DNA microsatellite-based method of analysis to allow traceability of the three Italian Protected Designation of Origin (PDO) table olives in comparison with fruits of another seven highly diffused table olive cultivars. The analyses were carried out by using 16 primer pairs, with a mean of five different alleles detected per primer set, and power of discrimination from 0.56 to 0.90. Allelic error rates in the range of 0鈥?.8% were observed. By combining data from the most reliable and highly informative microsatellites (DCA3, DCA16, DCA17, DCA18, UDO-043, and GAPU101), it was possible to identify the PDO fruits over the panel of 10 cultivars, with the probability of a chance match between different cultivars as low as 10鈥? and with 0.5% error rate. The amplification profile is independent of environmental and processing conditions and is helpful to verify the authenticity of PDO samples.