Probing Interactions between 尾-Glucan and Bile Salts at Atomic Detail by 1H鈥?sup>13C NMR Assays
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- 作者：Mette Skau Mikkelsen ; Sofia Bolvig Cornali ; Morten G. Jensen ; Mathias Nilsson ; Sophie R. Beeren ; Sebastian Meier
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2014
- 出版时间：November 26, 2014
- 年：2014
- 卷：62
- 期：47
- 页码：11472-11478
- 全文大小：352K
- ISSN：1520-5118
文摘
Polysaccharides are prospective hosts for the delivery and sequestration of bioactive guest molecules. Polysaccharides of dietary fiber, specifically cereal (1 鈫?3)(1 鈫?4)-尾-glucans, play a role in lowering the blood plasma cholesterol level in humans. Direct host鈥揼uest interactions between 尾-glucans and conjugated bile salts are among the possible molecular mechanisms explaining the hypocholesterolemic effects of 尾-glucans. The present study shows that 1H鈥?sup>13C NMR assays on a time scale of minutes detect minute signal changes in both bile salts and 尾-glucans, thus indicating dynamic interactions between bile salts and 尾-glucans. Experiments are consistent with stronger interactions at pH 5.3 than at pH 6.5 in this in vitro assay. The changes in bile salt and 尾-glucan signals suggest a stabilization of bile salt micelles and concomitant conformational changes in 尾-glucans.