An ICT-Based Approach to Ratiometric Fluorescence Imaging of Hydrogen Peroxide Produced in Living Cells

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• 作者：Duangkhae Srikun ; Evan W. Miller ; Dylan W. Domaille ; Christopher J. Chang
• 刊名：Journal of the American Chemical Society
• 出版年：2008
• 出版时间：April 9, 2008
• 年：2008
• 卷：130
• 期：14
• 页码：4596 - 4597
• 全文大小：135K
• 年卷期：v.130,no.14(April 9, 2008)
• ISSN：1520-5126

文摘

We present the synthesis, properties, and biological applications of Peroxy Lucifer 1 (PL1), a new fluorescent probe for imaging hydrogen peroxide produced in living cells by a ratiometric response. PL1 utilizes a chemoselective boronate-based switch to detect hydrogen peroxide by modulation of internal charge transfer (ICT) within a 1,8-naphthalimide dye. PL1 features high selectivity for hydrogen peroxide over similar reactive oxygen species, including superoxide, and nitric oxide, and a 65 nm shift in emission from blue-colored fluorescence to green-colored fluorescence upon reaction with peroxide. Two-photon confocal microscopy experiments in live macrophages show that PL1 can ratiometrically visualize localized hydrogen peroxide bursts generated in living cells at immune response levels.