Experimental Evidence of Weak Excluded Volume Effects for Nanochannel Confined DNA

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• 作者：Damini Gupta ; Jeremy J. Miller ; Abhiram Muralidhar ; Sara Mahshid ; Walter Reisner ; Kevin D. Dorfman
• 刊名：ACS Macro Letters
• 出版年：2015
• 出版时间：July 21, 2015
• 年：2015
• 卷：4
• 期：7
• 页码：759-763
• 全文大小：307K
• ISSN：2161-1653

文摘

We present experimental demonstration that weak excluded volume effects arise in DNA nanochannel confinement. In particular, by performing measurements of the variance in chain extension as a function of nanochannel dimension for effective channel sizes ranging from 305 to 453 nm, we show that the scaling of the variance in extension with channel size rejects the de Gennes scaling 未²X 鈭?D^1/3 in favor of 未²X 鈭?D⁰ using uncertainty at the 95% confidence level. We also show how simulations and confinement spectroscopy can be combined to reduce molecular weight dispersity effects arising from shearing, photocleavage, and nonuniform staining of DNA.