Poly(ethylene glycol)-Functionalized Polymeric Microchips for Capillary Electrophoresis
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- 作者：Xuefei Sun ; Dan Li ; Milton L. Lee
- 刊名：Analytical Chemistry
- 出版年：2009
- 出版时间：August 1, 2009
- 年：2009
- 卷：81
- 期：15
- 页码：6278-6284
- 全文大小：231K
- 年卷期：v.81,no.15(August 1, 2009)
- ISSN：1520-6882
文摘
Recently, we reported the synthesis, fabrication, and preliminary evaluation of poly(ethylene glycol) (PEG)-functionalized polymeric microchips that are inherently resistant to protein adsorption without surface modification in capillary electrophoresis (CE). In this study, we investigated the impact of cross-linker purity and addition of methyl methacrylate (MMA) as a comonomer on CE performance. Impure poly(ethylene glycol) diacrylate (PEGDA) induced electroosmotic flow (EOF) and increased the separation time, while the addition of MMA decreased the separation efficiency to approximately 25% of that obtained using microchips fabricated without MMA. Resultant improved microchips were evaluated for the separation of fluorescent dyes, amino acids, peptides, and proteins. A CE efficiency of 4.2 × 104 plates for aspartic acid in a 3.5 cm long microchannel was obtained. Chiral separation of 10 different d,l-amino acid pairs was obtained with addition of a chiral selector (i.e., β-cyclodextrin) in the running buffer. Selectivity (α) and resolution (Rs) for d,l-leucine were 1.16 and 1.64, respectively. Good reproducibility was an added advantage of these PEG-functionalized microchips.