Hoechst-IR: An Imaging Agent That Detects Necrotic Tissue in Vivo by Binding Extracellular DNA
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- 作者：Madhuri Dasari ; Sungmun Lee ; Jay Sy ; Dongin Kim ; Seungjun Lee ; Milton Brown ; Michael Davis ; Niren Murthy
- 刊名：Organic Letters
- 出版年：2010
- 出版时间：August 6, 2010
- 年：2010
- 卷：12
- 期：15
- 页码：3300-3303
- 全文大小：220K
- 年卷期：v.12,no.15(August 6, 2010)
- ISSN：1523-7052
文摘
Cell necrosis is central to the progression of numerous diseases, and imaging agents that can detect necrotic tissue have great clinical potential. We demonstrate here that a small molecule, termed Hoechst-IR, composed of the DNA binding dye Hoechst and the near-infrared dye IR-786, can image necrotic tissue in vivo via fluorescence imaging. Hoechst-IR detects necrosis by binding extracellular DNA released from necrotic cells and was able to image necrosis generated from a myocardial infarction and lipopolysaccharide/d-galactosamine (LPS-GalN) induced sepsis.