Analytical Characterization of the Role of Phospholipids in Platelet Adhesion and Secretion

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• 作者：Secil Koseoglu ; Audrey F. Meyer ; Donghyuk Kim ; Ben M. Meyer ; Yiwen Wang ; Joseph J. Dalluge ; Christy L. Haynes
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：January 6, 2015
• 年：2015
• 卷：87
• 期：1
• 页码：413-421
• 全文大小：398K
• ISSN：1520-6882

文摘

The cellular phospholipid membrane plays an important role in cell function and cell鈥揷ell communication, but its biocomplexity and dynamic nature presents a challenge for examining cellular uptake of phospholipids and the resultant effects on cell function. Platelets, small anuclear circulating cell bodies that influence a wide variety of physiological functions through their dynamic secretory and adhesion behavior, present an ideal platform for exploring the effects of exogenous phospholipids on membrane phospholipid content and cell function. In this work, a broad range of platelet functions are quantitatively assessed by leveraging a variety of analytical chemistry techniques, including ultraperformance liquid chromatography鈥搕andem electrospray ionization mass spectrometry (UPLC鈥揗S/MS), vasculature-mimicking microfluidic analysis, and single cell carbon-fiber microelectrode amperometry (CFMA). The relative enrichments of phosphatidylserine (PS) and phosphatidylethanolamine (PE) were characterized with UPLC鈥揗S/MS, and the effects of the enrichment of these two phospholipids on both platelet secretory behavior and adhesion were examined. Results show that, in fact, both PS and PE influence platelet adhesion and secretion. PS was enriched dramatically and decreased platelet adhesion as well as secretion from 未-, 伪-, and lysosomal granules. PE enrichment was moderate and increased secretion from platelet lysosomes. These insights illuminate the critical connection between membrane phospholipid character and platelet behavior, and both the methods and results presented herein are likely translatable to other mammalian cell systems.