Antibody- and Label-Free Phosphoprotein Sensor Device Based on an Organic Transistor

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• 作者：Tsukuru Minamiki ; Tsuyoshi Minami ; Petr Koutnik ; Pavel Anzenbacher Jr. ; Shizuo Tokito
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：January 19, 2016
• 年：2016
• 卷：88
• 期：2
• 页码：1092-1095
• 全文大小：343K
• ISSN：1520-6882

文摘

An antibody- and label-free detection of a phosphoprotein (α-casein) has been achieved using an organic field-effect transistor (OFET)-based sensor. The fabricated OFET device possesses an extended-gate electrode functionalized with an artificial phosphoprotein receptor (Zn^II-dipicolylamine complex, Zn^II-DPA). It is shown that the OFET responds to the molecular recognition processes involving the Zn^II-DPA at the extended-gate electrode. The binding of α-casein to the receptor in a HEPES solution results in an analyte-specific changes of the drain current of the OFET. The successful demonstration of the antibody- and label-free detection using the OFET could pave the way to the development of low-cost, disposable, and portable electronic sensor devices.