Multiple Oxidative Modifications in the Ophiobolin Biosynthesis: P450 Oxidations Found in Genome Mining

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• 作者：Koji Narita ; Ryota Chiba ; Atsushi Minami ; Motoichiro Kodama ; Isao Fujii ; Katsuya Gomi ; Hideaki Oikawa
• 刊名：Organic Letters
• 出版年：2016
• 出版时间：May 6, 2016
• 年：2016
• 卷：18
• 期：9
• 页码：1980-1983
• 全文大小：339K
• 年卷期：0
• ISSN：1523-7052

文摘

Heterologous expression of four candidate genes found in ophiobolin gene clusters from three fungal strains was employed to elucidate the late-stage biosynthetic pathway of phytotoxin ophiobolin. Expression of oblB_Ac (cytochrome P450) from the cryptic gene cluster gave unexpected products, and that of oblB_Bm/oblB_Ev from the gene cluster of ophiobolin producers, with oblD_Bm as the transporter, yielded intermediate ophiobolin C through an unusual four-step oxidation process. The observation made in this study may provide a useful guideline for the elucidation of genuine biosynthetic pathways of natural products.